Research Group 5: Reproduction Management

Electron microscopy

The electron microscopy (EM) is a central unit within the IZW open to all members of the institute as well as for cooperations with external scientists.

 A transmission electron microscope (TEM 902A, Zeiss) with an acceleration voltage of 80 kV and a maximum magnification of x250000 is available as well as a scanning electron microscope (DSM 940A, Zeiss) with an acceleration voltage of 15 kV and a maximum magnification of x100.000. For specimen preparation an EM-lab is fully equipped with Ultratrim, Ultracut, critical point drier, sputter coater and additionally there is a photography lab with enlarger and photo developer. An experienced EM-technician is taking care of the technical part, while on of the veterinary pathologists is responsible for the scientific aspects of the EM-unit.

                                                                                               
                                                        TEM 902A                                                                                                    DSM 940A

 Routinely ultrathin sections are made from resin embedded tissues as well as negative staining for the detection of virus particles from tissue homogenates or fecal samples. But also immuno-EM preparations are performed. Specimen for scanning electron microscopy require critical point drying and sputtering with heavy metals i.e. gold palladium.

The spectrum of different specimen preparations and investigations mirror the multi-faceted projects within the IZW, while the majority of samples comprise examinations for sperm morphology of various wild animals and ultrastructural investigations in pathology.   

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