 Methods
1. Ultrasonographic imaging
[devices
and range of application]
2. Computer tomography
[devices
and range of application]
3. (Videochip)
- Endoscopy [devices
and range of application]
4. Electron microscopy
[devices
and range of application]
5. Bright field microscopy
[devices
and range of application]
6. Anatomic
preparations [devices
and range of application]
7. genderspecific
sorting of spermatozoa [devices and range of application]
8. freezing techniques for germ cell
preservation [devices and range of application]
Ultrasonographic
imaging
Imaging modi of different ultrasound maschines:
- Color-Doppler-Flow (imaging of fluid movements)
- High-Frequency-Resolution (imaging of very small structures
(minimum 50µm) close to the surface)
- 3D (imaging of 3-dimensional correlations of structures,
fixed image mode)
- 4D (imaging of 3-dimensional correlations of structures,
moving image mode)
range of application:
- diagnosis of the sexual cycle
- diagnosis and monitoring of pregnancy
- diagnosis of gender in reptiles
- recording and retrospective analysis of measurements of
physiological and pathological structures (Imaging Archive)
The datasets of the analyses by ultrasonographic imaging
contribute to the imaging
archive.
Computer
tomography
Imaging modi:
- 2-dimensional imaging of the digital datasets ("virtual
")
- 3-dimensional imaging of the digital datasets ("free
rotation in 3-dimensional space")
- virtual passage through hollow systems (i.e. respiratory
system)
Range of application:
- imaging of topographic relationships of selected structures/
organs on live animals
- comparative analysis of anatomic structures (i.e. comparison
of the vocal tract in different mammal species)
- archiving of ct-data and retrospective analysis (Imaging
Archive)
The digital datasets of the analyses by computed tomography
contribute to the imaging
archive.
(Videochip)
- Endoscopy
Range of application:
- artificial insemination
- minimal-invasive Oocyte-retrieval in Rhinoceros species
- non-chirurgical imaging of the internal reproductive organs
of elephants
- obtaining of saliva of the parotid gland of ruminants
Electron
microscopy
Imaging modi:
- TEM - Transmission Electron Microscopy (imaging of 2-dimensional
section planes)
- SEM - Scanning Electron Microscopy (3-dimensional imaging)
Range of application:
- imaging of the speciesspecific morphology of spermatozoa
- testing the integrity of cryopreserved spermatozoa after
thawing
electron
microscopy
at the IZW
image
gallery
Bright field microscopy
Imaging modi:
- Phase-contrast-microscopy
- Fluorescence-microscopy
Range of application:
- Analysis of semen quality parametersUntersuchungen der Ejakulatqualität:
- Motility
- Concentration
- Morphology
- Examination of histological sections
Anatomic
preparations
Imaging modi:
- preparation of bony structures
- preparation of soft structures (muscles, neural structures,
vascular system)
Range of application:
- imaging of pathological structures
- comparative analysis of anatomic structures (inter and intraspecific)
- clarify evolutionmorphological interrelationships
Following archives were created:
- comparative skull and skeletal archive
genderspecific
sorting of spermatozoa
freezing techniques
for germ cell preservation
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