BioRescue – Advanced reproductive technologies for saving critically endangered mammals like the northern white rhinoceros

Only two Northern white rhinos are left in the world, both are females. Can we still save these animals from extinction? Together with international partners from science and conservation the BioRescue consortium aims at making the seemingly impossible a reality by developing advanced methods of assisted reproduction (aART) and stem cell associated techniques (SCAT).

Photo: Justin Mott
Project details
Duration: 05/2019 – 04/2022
Third-party funded: yes
Involved Department(s): Dept Reproduction Management, Dept Evolutionary Genetics
Leibniz-IZW Project Leader(s): Thomas Hildebrandt (Dept Reproduction Management)
Leibniz-IZW Project Team:

Frank Göritz, Robert Hermes, Susanne Holtze, Pierfrancesco Biasetti, Daniel Čižmár, Charlotte Okolo (all: Dept Reproduction Management), Steven Seet, Jan Zwilling, Antje Queißner (all: Science Management), Arne Ludwig (Dept Evolutionary Genetics)

Consortium Partner(s):

Max Delbrück Center for Molecular Medicine (MDC), AVANTEA Laboratory of Reproductive Technologies, Safari Park Dvůr Králové, Universita degli studi di Padua, Kyushu University

Current Funding Organisation: German Federal Ministry of Education and Research (BMBF) (3.0 Million Euros)
Research Foci:
Improving population viability
Developing theories, methods, and tools
Videos about the project
Photo galleries


In light of the Earth’s sixth great extinction event driven by man traditional conservation strategies such as habitat protection and ex-situ breeding combined with reintroduction programs will not be sufficient to stop or even to slow down this process significantly. Currently, 22% of the mammals are at risk of extinction. The family Rhinocerotidae is particularly affected, with three of the five extant species listed as critically endangered (Sumatran, Javan, and black rhinoceros), one listed as vulnerable (greater one-horned rhinoceros), and one, the southern white rhinoceros (SWR, Ceratotherium simum simum), listed as near threatened. Contrarily, in 2008 the IUCN officially declared the northern counterpart of the SWR, the northern white rhinoceros (NWR, Ceratotherium simum cottoni) as extinct in the wild. On March 19th, 2018 the death of the last male NWR, Sudan, brought wide public attention to the doomed fate of this subspecies and served as a clarion call for alternative measures of conservation.

The long-term consequences of the loss of a keystone species for the fragile ecosystem of Central Africa are not fully predictable. However, the eradication of a megavertebrate and important landscape architect such as the NWR will cause at least a significant disturbance or even destruction to elements of the complex ecosystem. The loss of a keystone species can initiate a so called “vortex effect”. The term was coined by Bob Lacey (1993) and used to name his famous Vortex Population Viability Analysis software, distributed freely by the IUCN. The vortex effect stands for the accelerated loss of species and entire species societies whose life history directly or indirectly depends on keystone species which are on the brink of extinction.

In 2015 a group of 20 international scientists from five continents met in Vienna to develop a new strategic roadmap (Saragusty et al., 2016) for saving the critically endangered NWR. At that time only three individuals (1 male, 2 females) of this subspecies were alive. The new approach combines advanced assisted reproductive technologies (aART) and stem cell associated techniques (SCAT). This combined third strategy, in addition to the two established main strategies of habitat protection and classical ex-situ conservation programs, allows for the employment of biomaterial of live and deceased individuals in form of cryopreserved gametes (Hermes et al., 2018) as well as of skin samples for fibroblast cultures. Fibroblast cultures via induced pluripotent stem cell transformation can subsequently be used for the in vitro production of artificial gametes. This third way of species protection is now being developed in the international BioRescue project and put into practice immediately.

The NWR is the ideal role model for this innovative approach due to the existence of cryopreserved biomaterial of deceased individuals. There are 12 NWR fibroblast cell lines representing eight presumably unrelated founders (Tunstall et al., 2018) and approx. 300 ml of cryopreserved semen from four different NWR bulls (Hermes et al., unpubl. data). This precious biomaterial together with the two living NWR females Najin and Fatu as potential oocyte donors represent the pillars of our new strategy. The production of healthy embryos derived from natural gametes (aART) and from cell culture generated artificial gametes (SCAT) will allow for the preservation of the NWR genome and the subsequent embryo transfer into surrogates using female SWR.

The work packages (WP) of the project focus on the development of suitable technologies and protocols to rescue the NWR and to provide the opportunity to establish a self-sustaining, genetically healthy NWR population which can be reintroduced to the wild. In addition to the two approaches to embryo production, an ethical risk analysis as a separate work package is part of the project. If innovative research shifts the boundaries of what is possible in species protection, new risks that have not yet been adequately evaluated will emerge on the one hand, and ethical questions on the other, which will bring together the well-being of individuals, the prosperity of an entire subspecies and complex social-ecological questions. These new risks and ethical questions are systematically analysed and discussed in the BioRescue project, also involving relevant stakeholders and the interested public in the discourse.

The German Federal Ministry of Education and Research (BMBF) supports the German partners of the BioRescue Consortium. At the IZW the project is conducted within the Department of Reproduction Management, supplemented by genetical assessments by the Department of Evolutionary Genetics.


Selected Publications


Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C (2018): Embryos and embryonic stem cells from the white rhinoceros. NAT COMMUN 9, 2589. doi:10.1038/s41467-018-04959-2.

Hermes R, Hildebrandt TB, Göritz F (2018): Cryopreservation in rhinoceros - setting a new benchmark for sperm cryosurvival. PLOS ONE 13, e0200154. doi:10.1371/journal.pone.0200154.

Saragusty J, Diecke S, Drukker M, Durrant B, Ben-Nun I, Galli C, Göritz F, Hayashi K, Hermes R, Holtze S, Johnson S, Lazzari G, Loi P, Loring JF, Okita K, Renfree MB, Seet S, Voracek T, Stejskal J, Ryder OA, Hildebrandt TB (2016): Rewinding the process of mammalian extinction. ZOO BIOL 35, 280-292. doi:10.1002/zoo.21284.

Saragusty J, Osmers J-H, Hildebrandt TB (2016): Controlled ice nucleation - is it really needed for large-volume sperm cryopreservation? THERIOGENOLOGY 85, 1328-1333. doi:10.1016/j.theriogenology.2015.12.019.

Hermes R, Schwarzenberger F, Göritz F, Oh S, Fernandes T, Bernardino R, Leclerc A, Greunz E, Mathew A, Forsyth S, Saragusty J, Hildebrandt TB (2016): Ovarian down regulation by GnRF vaccination decreases reproductive tract tumour size in female white and greater one-horned rhinoceroces. PLOS ONE 11, e0157963. doi:10.1371/journal.pone.0157963.

Arav A, Saragusty J (2016): Directional freezing of sperm and associated derived technologies. ANIM REPROD SCI 169, 6-13. doi:10.1016/j.anireprosci.2016.02.007.

Prieto MT, Sanchez-Calabuig MJ, Hildebrandt TB, Santiago-Moreno J, Saragusty J (2014): Sperm cryopreservation in wild animals. EUR J WILDL RES 60, 851-864. doi:10.1007/s10344-014-0858-4.

Galateanu G, Hermes RSaragusty J, Göritz F, Potier R, Mulot B, Maillot A, Etienne P, Bernardino R, Fernandes T, Mews J, Hildebrandt TB (2014): Rhinoceros Feet Step Out of a Rule-of-Thumb: A Wildlife Imaging Pioneering Approach of Synchronized Computed Tomography-Digital Radiography. PLOS ONE 9, e100415. doi:10.1371/journal.pone.0100415.

Arav A, Saragusty J (2014): Directional freezing of spermatozoa and embryos. REPROD FERTIL DEV 26, 83-90. doi:10.1071/Rd13295.