Dr. Mohammad Bashawat

• New fluorescent cholesterol analogs for cell biological research: synthesis, characterization,
  and application.
• Improvement of cryopreservation of dissociated testis cells and spermatozoa in the presence
  of kinase inhibitors.
• Cryopreservation of male gametes – optimization using lipid additives

• Cryopreservation of sperm, testicular cells, and testicular tissue.
• 3 D cell culture systems and in vitro models of spermatogenesis.
• Bioimaging, image analysis, and automated image segmentation pipelines for reproductive
  and cellular biology.
• Machine-learning strategies for the characterization of mammalian testicular cells.

1. Thaysen K., Lehmann M., Akkerman V., Bashawat M., Reinholdt P., Leopold J., Jürgen S., Scheidt H., Kongsted J., Sperlich E., Müller P., Wüstner D (2026). Sterol trafficking in yeast studied by one-and twophoton live-cell imaging of an intrinsically fluorescent ergosterol analog. Methods and Applications in Fluorescence., 14 025009. https://doi.org/10.1088/2050-6120/ae5e19

2. Bashawat M., Schulze M., Müller P., Müller K., (2025) Differential impact of the kinase inhibitors ruxolitinib and ceritinib on porcine sperm in vitro. Animal Reproduction Science., 277, 107850. https://doi.org/10.1016/j.anireprosci.2025.107850

3. Max L., Halder S., Reinholdt P., Bashawat M., Scheidt H., Leopold J., Schiller J., Di Prima D., Akkerman V., Szomek M., Lauritsen L., Kongsted J., Müller P., Wessig P., Wüstner D. (2024) Synthesis and Characterization of a Novel Intrinsically Fluorescent Analog of Cholesterol with Improved Photophysical Properties. Analytical Chemistry., 96, 47, 18596–18604. https://doi.org/10.1021/acs.analchem.3c05720

4. To H., Reinholdt P., Bashawat M., Luck M., Lauritsen L., Akkerman V., Kroiss M., Wüstner D., Kongsted J., Müller P., Scheidt H. (2024) The impact of acyl-CoA: cholesterol transferase (ACAT) inhibitors on biophysical membrane properties depends on membrane lipid composition. Molecular and Cellular Endocrinology., 112385. https://doi.org/10.1016/j.mce.2024.112385

5. Fischer M., Luck M., Werle M., Vogel A., Bashawat M., Ludwig K., Scheidt H. A., Müller P. (2023) The smallmolecule kinase inhibitor ceritinib, unlike imatinib, causes a significant disturbance of lipid membrane integrity: A combined experimental and MD study. Chemistry and Physics of Lipids., 257, 105351. https://doi.org/10.1016/j.chemphyslip.2023.105351

6. Akkerman V., Scheidt H. A., Reinholdt P., Bashawat M., Szomek M., Lehmann M., Wessig P., Covey D.F., Kongsted J., Müller P., Wüstner D. (2023) Natamycin interferes with ergosterol-dependent lipid phases in model membranes. BBA advances., 4, 100102. https://doi.org/10.1016/j.bbadva.2023.100102

7. Bashawat M., Braun B.C., Müller K., Hermann B.P. (2023) Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development–A model for wild felids. Theriogenology Wild., 2, 100031. https://doi.org/10.1016/j.therwi.2023.100031

8. Bashawat M., Hensel B., Müller K., Schulze M. (2021) Cooled storage of semen from livestock animals (Part II): Camelids, Goat and Sheep. Animal Reproduction Science., 234, 106855. https://doi.org/10.1016/j.anireprosci.2021.106855

9. Müller K., Eder S, Jakop U., Schiller J., Müller P., Bashawat M. (2020) Assisted reproduction for felid species conservation– sperm competences at risk. Reproduction in Domestic Animals., 55(suppl. 2): 55-60. https://doi.org/10.1111/rda.13581

10. Bashawat M., Braun B.C., Müller K. (2020) Cell survival after cryopreservation of dissociated testicular cells from feline species. Cryobiology., 97: 191-197. https://doi.org/10.1016/j.cryobiol.2020.03.001

11. Bashawat M., Al-Merestani M. R., Moussa M., Tainturier D. (2018) Combined Amino Acid Proline with LDL fractions in Awassi Rams Semen Extenders. The Arab Journal for Arid Environments., 11 (1-2).

12. Bashawat M., Moussa M., Al-Merestani M. R., Destrumelle S., Tainturier D. (2016) The use of different lowdensity lipoproteins concentrations for developing a new extender for Awassi ram's semen. Scientific Journal

of King Faisal University (Basic and Applied Sciences)., 17 (1), 1437H.

13. Bashawat M., Moussa M., Al- Merestani M. R., Tainturier D. (2016) Effect of different extenders on Awassi rams frozen semen fertility. The Arab Journal for Arid Environments., 9 (1-2).